OVERVIEW OF TECHNOLOGIES
1D and 2D-PAGE
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Classical (silver, Colloidal Coomassie blue)
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Fluorescent protein stains (DIGE using Cy™ dyes)
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Post Translational Modification specific (eg Pro-Q Diamond)
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Gel Image Analysis
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Image scanner Labscan
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Image Master 2D Platinum
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Decyder v7.0
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Spot Excision/ Enzymatic Digestion of Proteins
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Mass Spectrometric analysis
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Peptide mass fingerprinting MALDI TOF MS
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Peptide fragments fingerprint ESI-IT
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Sequence analysis 4800 MALDI TOF/TOF
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Post-translation modification analysis- phosphorylation, acetylation
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iTRAQ and LC-MS/MS workflows for protein identification and relative quantification.
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Bioinformatics
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Mass spectrometry data analysis
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Data storage
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